(cytology) A loop of double-stranded DNA that is separate from — and replicates independently of — the chromosomes; such loops are most commonly found in bacteria, but they are also sometimes found in archaeans and eukaryotic cells, and they are used in genetic engineering as a vector for gene transfer.
Used in a Sentence
plasmid
Definition, parts of speech, synonyms, and sentence examples for plasmid.
Editorial note
Because the batch number for our competent cells from NEB changed and it no longer accepts our plasmid.
Examples15
Definitions1
Parts of speech1
Quick take
(cytology) A loop of double-stranded DNA that is separate from — and replicates independently of — the chromosomes; such loops are most commonly found in bacteria, but they are also sometimes found in archaeans and eukaryotic cells, and they are used in genetic engineering as a vector for gene transfer.
Meaning at a glance
The clearest senses and uses of plasmid gathered in one view.
Definitions
Core meanings and parts of speech for plasmid.
noun
(cytology) A loop of double-stranded DNA that is separate from — and replicates independently of — the chromosomes; such loops are most commonly found in bacteria, but they are also sometimes found in archaeans and eukaryotic cells, and they are used in genetic engineering as a vector for gene transfer.
Example sentences
Because the batch number for our competent cells from NEB changed and it no longer accepts our plasmid.
My slightly informed opinion about biotech consumables is that a plasmid construct is only half of the deal.
However, there did not seem to be much/any effort to design their plasmid to minimise these off target mutations.
With these plasmid being traded around out in the wild, the fear is that we might soon see all beta-lactams become useless, like penicillin is now.
However, that does not exclude the possibility of the bacterium acquiring a plasmid or phage carrying an enzyme that inactives teixobactin.
I'm not sure though if human cells will express plasmid vectors, though I know some eukaryotic cells do).
From the first time I cloned a gene (that had already been isolated, and stored in a plasmid) into E.
Depending on the composition of the plasmids, it should be very straight forward, and presumably the manufacturer will also provide plasmid-specific protocols to help.
By the same notion, it's not considered artificial life when a new custom chromosome (called a plasmid) is inserted into a bacteria or eukaryotic cell.
As I posted earlier: > there did not seem to be much/any effort to design their plasmid to minimise these off target mutations.
I've seen it used in an application that manages DNA plasmid data, it could be useful in a lot of genomic analysis tools.
So for a plasmid to encode resistance to this new antibiotic, it would need to contain the code for a protein which disrupts the action for the antibiotic.
Quote examples
Bacteria will "eject" a plasmid within a generation or two if they no longer need the gene(s) in question.
DNA origami is actually surprising simple; the design is not at all "as complicated as it sounds." Short, synthesized strands of DNA that are complementary to various bits of a long scaffold strand of DNA (often single-stranded plasmid DNA, like M13mp18) in a manner similar to the way one pushes different faces of paper together with paper origami.
Since bacterial plasmid propagation is "error-free", we don't bother sequencing all but 1kb or so of the final product - saves on cost - and our plan is to do shotgun sequencing of all of the plasmids, barcoded, with ionTorrent when we're done.
Frequently asked questions
Short answers drawn from the clearest meanings and examples for this word.
How do you use plasmid in a sentence?
Because the batch number for our competent cells from NEB changed and it no longer accepts our plasmid.
What does plasmid mean?
(cytology) A loop of double-stranded DNA that is separate from — and replicates independently of — the chromosomes; such loops are most commonly found in bacteria, but they are also sometimes found in archaeans and eukaryotic cells, and they are used in genetic engineering as a vector for gene transfer.
What part of speech is plasmid?
plasmid is commonly used as noun.