luciferase

Somehow fixing the release of luciferase enzymes to dark conditions only (currently the glowing is always active).

There's been studies done with luciferase to see where the mRNA triggers protein production in small animals.

Specifically: sure, it you wanted to feed a pile of ATP to some luciferase to output photons.

It’s kind of like saying a luciferase based assay is just measuring the intensity of light.

Then by measuring light emitted by the luciferase, you get an indirect measure of the level of the target protein.

It's not trivial to generate the necessary luciferin/luciferase, but adding GFP is a basic project.

Note that we have animal studies that used luciferase mRNA to see where mRNA medications are transported and translated.

A common use is to engineer a luciferase into a genome so that it is expressed in conjunction with a particular protein that you’re interested in.

Luciferin is converted to a reactive intermediate by the enzyme luciferase in a reaction that uses ATP (produced by mitochondria), this intermediate reacts with O2 to produce light.

Good remark but we were thinking of luciferase outside the cellular context.

We expect that the reason why ostracod luciferase was exploited for kleptoprotein bioluminescence of Parapriacanthus is due partly to its high proteolytic resistance and highly stable nature (fig.

Exactly, I once did the calculation, they use the protein luciferase, which requires 1 molecule of Lucferin and 1 molecule of ATP per photon produced!

We were looking into "bio-light" using luciferase (the mentioned firefly protein).

But arguable keeping “naked” luciferase functional is as well… For cells you need a lot more than just glucose, you needs some medium and fetal bovine serum and you need a way to remove waste and dead cells, etc.

Luciferase isn't the scary thing she's talking about at all - it's HOW you see the results from a PCR test.

I heard about Luciferase[0] a class of oxidative enzymes that produce bioluminescence.